Figure 3

Overexpression of the yidC mRNA transcripts and subsequent effect on the YidC protein synthesis in mycobacteria. Cultures of M. smegmatis harbouring pTetR::yidC(Msm) or pTetR::yidC(Mtb) were treated with different concentrations of ATc (0–50 ng/ml) for 24 hours and copy number of respective mRNA transcripts was measured by qRT-PCR using standards prepared with known concentrations of gnomic DNA, as described earlier¹⁷ (A). Effect of overexpression of mRNA transcripts of both yidC(Msm) and yidC(Mtb) on the levels of the respective YidC proteins was examined, in M. smegmatis (B) or in M. tuberculosis (C). Shown are the envelope proteins of bacteria (20 µg/lane) in CBB-stained denaturing polyacrylamide gels. Mean ± s.d. of three independent experiments is shown in (A). Asterisks represent level of significance as determined by paired Student’s t-test (P < 0.05). Arrowheads mark the envelope proteins that are altered upon YidC(Mtb) induction. Images in (B-C) represent 3 biological replicates.