Figure 5

Influence of YidC-overexpression on global gene expression profile of M. tuberculosis. Heat map shows the status of differentially expressed genes in the microarray analysis of M. tuberculosis::pTetR-yidC(Mtb) compared to control after 4 days of treatment with 50 ng/ml ATc (A). Whole-genome transcriptional analysis was performed by microarray hybridization using arrays specific to M. tuberculosis H37Rv (Microarray Inc., USA). Increasing expression levels are denoted by different colours ranging from red to pink, respectively. Genes that exhibit >2-fold difference in expression between the two strains consistently in three biological replicates with P values of ≤ 0.05 and FDR of ≤ 5% are included in (A). As shown, overexpression of yidC(Mtb) results in induction of cell surface stress regulons, known to respond to cell surface stresses like SDS, indicating overexpression of yidC(Mtb) affects cell envelope integrity of M. tuberculosis. Differential expression of select genes including those regulated by SDS-treatment¹⁶, was also determined by qRT-PCR using RNA samples prepared from M. tuberculosis expressing yidC(Mtb) or yidC(Msm) and expression values in yidC-overexpressing cells relative to control are plotted for a comparative analysis (B). Mean ± s.d. of three independent experiments is shown. Asterisks represent level of significance as determined by paired Student’s t-test (P < 0.05).