Figure 6
From: Regulation and overexpression studies of YidC in Mycobacterium tuberculosis
Modulation in YidC expression perturbs integrity of mycobacterial cell envelope. Relatively higher uptake of EtBr (A,B) and ruptured lipid bilayer (C,D) of bacteria overexpressing full length YidC(Mtb) compared to empty plasmid control or cells expressing YidC(Msm) indicate that YidC(Mtb) induction causes a membrane stress on bacteria. Induction of YidC was performed for 4 days in M. tuberculosis and for 24 hours in M. smegmatis. EtBr uptake was measured either for 15–60 mins in M. tuberculosis (A) or for 15 mins in M. smegmatis (B) cells overexpressing YidC, whereas cellular morphology was performed by transmission electron microscopy of M. tuberculosis (C). An enlarged image depicting portion of cell envelope of different strains is shown in (D). Mean ± s.d. of three independent experiments is shown in (A,B), whereas images in (C) represent 3 different fields, each from 2 biological replicates. Asterisks represent level of significance as determined by paired Student’s t-test (P < 0.05).
