Table 1 List of plasmids and primers.
From: Regulation and overexpression studies of YidC in Mycobacterium tuberculosis
A. Primers | |||
|---|---|---|---|
S. No. | Primer Name | Sequence (5′-3′) | Reference |
1. | Pr17 | GTTTGATTTCTTCAGTCTCG | 14(Forward primer for qRT-PCR analysis of yidC(Mtb)) |
2. | Pr18 | TTGATCTGTGGTTGCAGTTC | 14(Reverse primer for qRT-PCR analysis of yidC(Mtb)) |
3. | Pr3 | GATCCATATGAGTCTTTTGTTTGATTTC | 14(Forward primer for PCR amplification of yidC(Mtb) ORF) |
4. | Pr5 | GCGCAAGCTTTCAACGTTTGCGTTTTTTCGGTC | 17(Reverse primer for PCR amplification of yidC(Mtb) ORF) |
5. | rpmH-F | GATCCATATGACCAAGGGCAAAAGGACCTTCCAG | This study |
6. | Pr45 | ATGACGCAACGGTCCGGGTTG | This study |
7. | MRA_3959-JnR | CTAGTCGCGGAGCACAACG | This study |
8. | yidC-JnF | CCGAGCGCCCGTACGCCGCG | This study |
9. | MS6942_F | GCGCCATATGTTTAACTTCTTCAGCTTG | This study (For PCR amplification of yidC(Msm) ORF) |
10. | MS6942_R | GCGCAAGCTTTCATCGTTTCCGCTTCTTGG | |
11. | MS6942_RTF | AAGCTCGCGCTCTACGTGTTCC | This study (For qRT-PCR analysis of yidC(Msm)) |
12. | MS6942_RTR | TCATCCGATTCGGCTTGGCG | |
13. | Rv0251c_RTF | CAATCTCGCATTGTGGTCGC | This study (For qRT-PCR analysis of Rv0251c) |
14. | Rv0251c_RTR | TTGTCGACGTCAATGCCGGG | |
15. | Rv0341c_RTF | AGTGCCGGGCTGATCGATATCG | This study (For qRT-PCR analysis of Rv0341c) |
16. | Rv0341c_RTR | TCGTGATGACGCTTGCCACC | |
17. | Rv0676c_RTF | ATGATCGTGCAAAGGACAGC | This study (For qRT-PCR analysis of Rv0676c) |
18. | Rv0676c_RTR | ACACCGCCTGTATCTGTCCG | |
19. | Rv1221_RTF | CGGGTTGGGAATACGGAATCG | This study (For qRT-PCR analysis of Rv1221) |
20. | Rv1221_RTR | CCTGCAATTGGTCAGACGGC | |
21. | Rv1909_RTF | CCTCTATACCGGACTACGCC | This study (For qRT-PCR analysis of Rv1909) |
22. | Rv1909_RTR | AGGGTTGGATCTTTCGCACC | |
23. | Rv2590_RTF | TCGAGGACCTATACGCCAGC | This study (For qRT-PCR analysis of Rv2590) |
24. | Rv2590_RTR | AGTAGCTCGACCATGGTGCG | |
25. | Rv2710_RTF | GGGTTGACAGCGATCTGGATGC | This study (For qRT-PCR analysis of sigB) |
26. | Rv2710_RTR | GGTCGCGTTTTCGGTTCTCG | |
27. | Rv2744c_RTF | ATGGCCAATCCGTTCGTTAAAGC | This study (For qRT-PCR analysis of Rv2744c) |
28. | Rv2744c_RTR | GCATCTCCAATTGACGCTGG | |
29. | Rv3862c_RTF | CATGACAGTAACCGCCCTGT | This study (For qRT-PCR analysis of whiB6) |
30. | Rv3862c_RTR | TTCGGGAATTACGACCCCTG | |
B. Plasmids | |||
S. No. | Name | Plasmid description | Reference/Source |
1. | pGEM-T® Easy | Plasmid with E. coli Ori for TA Cloning | Promega |
2. | pGEM-yidC(Msm) | Derivative of pGEM- T® Easy harbouring MSMEG_6942 ORF | This study |
3. | pGEM-yidC(Mtb) | Derivative of pGEM- T® Easy harbouring MRA_3960 ORF | This study |
4. | pTetR | Replicative Mycobacteria- E. coli shuttle plasmid for ATc inducible expression of genes in mycobacteria | |
5. | pTetR-yidC(Msm) | Derivative of pTetR harbouring MSMEG_6942 ORF at Nde I-Hind III downstream to ATc-inducible promoter | This study |
6. | pTetR-yidC(Mtb) | Derivative of pTetR harbouring MRA_3960 ORF at Nde I-Hind III downstream to ATc-inducible promoter | This study |
