Figure 2
From: Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis
Immunodetection of AtDGAT3 in A. thaliana in germinating seeds. A. thaliana seeds (from the Col-0 accession) were stratified on humid Whatman discs at 4 °C in the dark for 72 hours and then transferred in a growth cabinet (25 °C, constant light) to trigger germination. Proteins were extracted from 10 seeds or seedlings during the germination process (0, 24, 48, or 72 h after the first exposure to the light), separated by SDS-PAGE, transferred and immunodetected using polyclonal antibodies raised in rat against Δ75AtDGAT3. The arrow indicates the localization of the protein (calculated MW = 39.2 kDa). Separations indicate delineation of the figure.
