Figure 3
From: Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis
SDS-PAGE and Western-blot analyses of recombinant AtDGAT3 proteins after purification by affinity chromatography. The full-length (40.2 kDa), Δ46 (35.4 kDa) and Δ75 (31.9 kDa) protein variants of recombinant His-tagged AtDGAT3 were purified on a nickel chelating resin from the 12 000 x g supernatant of the lysate of an E. coli culture and further purified using size exclusion chromatography (Δ46). Protein concentration was determined by the Bio-Rad assay and 7.5 to 10 µg were separated by SDS-PAGE. Each variant protein is indicated by a black arrow. The identity of the band corresponding to the full-length protein was validated by Western blot and by mass spectrometry. The band (highlighted with a black asterisk) of the SDS-PAGE was cut, digested and analysed by LC-MS/MS. Vertical lines indicate delineation of the figure. Original gels and blot are displayed in Supplementary Fig. S2A–C.
