Figure 4
From: Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis
AtDGAT3 UV-visible spectroscopy. (A) Freshly purified Δ46AtDGAT3 UV-visible spectrum was registered in aerobic conditions with 16,5 μM of protein on a Shimadzu UV-1800 spectrophotometer. The UV-Vis spectrum presents a typical oxidized [2Fe-2S]2+ cluster with sulfur to iron charge transfer absorption bands observed here at 335 nm, 425 nm, and 550 nm. (B) Δ46AtDGAT3 UV-visible spectrum of a freshly purified protein at 337 µM was recorded before (spectrum 1) and after addition of sodium dithionite at 2 mM (spectrum 2: 1 minute; spectrum 3: 30 min), or 100 mM (spectrum 4: 20 min).
