Figure 5

Pace analysis diagrams to determine protein thermal stability. Heat denaturation profiles (a and b) and standard Gibbs energy of unfolding plots (c and d) of 10 mM α-Lac in 20 mM Tris buffer (pH 7) were determined in the absence and presence of various concentrations of DOX or PTX. Excitation and emission wavelengths were 280 and 337 nm, respectively. α-Lac (●), α-Lac:DOX or α-Lac:PTX ratio of 1:2 (▲), and α-Lac:DOX or α-Lac:PTX ratio of 1:5.5 (♦).