Figure 1
From: Architecture and subunit arrangement of the complete Saccharomyces cerevisiae COMPASS complex
Overexpression and purification of the COMPASS complex from budding yeast S. cerevisiae. (A) COMPASS overexpression scheme. Full length SET1 and the Cps subunits of COMPASS (CPS60, CPS50, CPS40, CPS35, CPS30, and CPS25) together with GAL4 and GAL1-10 were cloned into pRS30-series vectors. A TAP tag was fused to the N-terminus of Set1. (B) SDS-PAGE analysis of the purified COMPASS complex. Purified Set1 consistently runs on SDS/PAGE as a doublet, the reasons behind this property of Set1 or its biological significance remain unclear7. (C) In vitro H3K4 mono-, di-, and tri-methylation activities of COMPASS complex, examined by western blotting using H3K4me1, me2, and me3 antibody and anti-H3 antibody.
