Figure 3
From: Architecture and subunit arrangement of the complete Saccharomyces cerevisiae COMPASS complex
Key subunit mapping and XL-MS analysis on COMPASS complex. (A–C) Identification of the key COMPASS subunits determined through a tag or Fab labeling strategy. 3D cryo-EM maps of COMPASS Set1–DID (A), COMPASS Cps50–PA–NZ-1 (B), and COMPASS Cps60–eGFP (C), with COMPASS Set1–DID and COMPASS Cps60–eGFP showing the front view of the structure, and COMPASS Cps50–PA–NZ-1 showing the back of COMPASS. The density attributed to COMPASS is shown in grey, and that of DID, NZ-1 Fab, and eGFP, in red, green, and blue, respectively. (D) XL-MS analysis on COMPASS complex. Identified cross-linked contacts between different subunits are shown as dotted lines. We used Best e-value (1.00E-01) as the threshold to remove extra XL-MS data with lower confidence.
