Figure 3
Inhibition of STAT3 reduces AR activity in PSAhi ENZR cells. (A) Relative mRNA expression of AR target genes PSA, NKX3-1 and FKBP5 and (B) Protein expression of p-STAT3S727, STAT3, PSA, AR and Vinculin in PSAhi ENZR cells 49 C and 49 F treated with 0, 5 or 10 µM GPA500 for 48 hours. (C) Protein expression of p-STAT3S727, STAT3, PSA, AR and Vinculin and (D) Relative AR activity assessed by luciferase assay and (E) Relative mRNA expression of AR target genes PSA, FKBP5, NKX3-1 and TMPRSS2 in LNCaP (Left) and 16D CRPC cells (Right) treated with 10 µM ENZ or GPA500 or combination of both for 48 hours. (F) Relative 72 hour cell proliferation assay using WST8 in LNCaP (Left) and 16D CRPC cells (Right) treated with indicated ENZ and GPA500 concentration. All graphs represent pooled data from 3 independent experiments and relative luciferase activity is compared to non-treated samples (Control = 1).
