Table 2 Wolbachia and Asaia infection prevalence rates in adult female mosquitoes using three conserved Wolbachia genes (16S rRNA, ftsZ and wsp).

From: Diverse novel resident Wolbachia strains in Culicine mosquitoes from Madagascar

Mosquito species

Number

Wolbachia gene PCR amplification

Prevalence (%)

Fisher’s exact post hoc P value (Wolbachia vs. Asaia)

16S rRNA

ftsZ

wsp

Wolbachia

Asaia

Aedes albocephalus

1

0

0

0

   

Aedes circumlateolus

1

0

0

0

 

100

(20–100)

 

Aedomyia furfuria

2

0

0

0

 

100

(34–100)

 

Aedomyia madagascarica^

9

9

9

9

100

(70–100)

89

(56–98)

>0.99

Culex antennatus^

32

1

1

0

3

(1–16)

56

(38–74)

>0.99

Culex bitaeniorhynchus

12

0

0

0

 

58

(32–81)

 

Culex decens^

17

3

3

3

18

(4–43)

18

(4–43)

0.46

Culex duttoni^

1

1

1

0

100

(21–100)

100

(21–100)

>0.99

Culex tritaeniorhynchus^

19

0

0

0

 

95

(74–100)

 

Culex giganteus

5

0

0

0

 

80

(37–96)

 

Culex pipiens complex

44

0

0

0

 

7

(1–30)

 

Culex poicilipes

21

0

0

0

 

48

(28–68)

 

Ficalbia circumtestacea

3

1

1

0

33

(6–79)

  

Mansonia uniformis^

24

7

7

0

29

(15–49)

67

(47–82)

0.65

Uranotaenia spp^

27

7

7

7

26

(13–45)

19

(8–37)

0.54

  1. An individual was considered Wolbachia-infected when any one of the three Wolbachia gene fragments were amplified. Species containing resident Wolbachia strains are in bold. Wolbachia and Asaia prevalence rates are shown with 95% confidence intervals in parentheses. Fisher’s exact post hoc test P value is shown comparing Wolbachia and Asaia infections in individual mosquitoes from species that contained at least one individual of both bacterial endosymbionts. ^ denotes where CO1 sequences were obtained for phylogenetic analysis of mosquito species.