Figure 7
Representative TUNEL assay of FUS focus area in excessive microbubble dose group. Rats were treated with one to three episodes of either 0.33-MI or 0.8-MI FUS-BBB opening. All were given 0.4 ml/kg of microbubbles prior to FUS application. In the 0.33-MI group, a weak positive TUNEL assay could be detected after the first sonication, but was not detected after the second and third episodes. In the 0.8-MI exposure group, a strong positive TUNEL assay could be seen after first sonication, and progressively increased after subsequent episodes. n = 6. Scale bar: 20 µm, Blue: DAPI. Green: TUNEL assay. Arrow: positive TUNEL assay.
