Figure 5

A03 by SQ delivery increases SirT1 in frontal cortex of male E4FAD mice. (a) After 28-day treatment of E4FAD mice with 10 mg/kg/day A03 or E1 by SQ injection, there was a trend (p = 0.0841) for an increase in SirT1 in the frontal cortex (FrCx) when all mice were analyzed together, but SirT1 levels were unchanged in mice treated with E1. In the ANOVA summary, F = 0.1958 and p = 0.8236. (b) When SirT1 levels in FrCx were analyzed by gender, the A03-treated male E4FAD mice showed a significant increase in SirT1 (p = 0.0424). Because there were fewer females, that group was likely under powered for statistics (n = 3 for NTg Veh, E4FAD Veh and A03 females, and n = 4 for E4FAD E1 females; n = 5 for all males in all groups except the E1 group, wherein n = 4). The ANOVA summary was F = 0.0833 and p = 0.9210 for females and F = 4.700 and p = 0.0335 for males. (c) There was no significant increase in SirT1 in the parietal cortex (PtCx) of A03 or E1-treated mice as compared to E4FAD Veh mice (AU = Arbitrary Units; n = 8 for all groups). In the one-way ANOVA summary, F = 0.1958 and p = 0.8236. (d) There was also no significant difference as a result of treatment when data were analyzed by gender. In the ANOVA summary, F = 0.2039 and p = 0.8202 for E4FAD females and F = 0.1990 and p = 0.8224 for E4FAD males (NTg mice were not compared). All statistical analysis was performed using one-way ANOVA with Tukey’s Multiple Comparison Test to compare E4FAD groups. Data graphed as the mean ± SEM.