Figure 8

A03 improves memory. NTg Veh and Veh or A03-treated E4FAD mice underwent Novel Object Recognition (NOR) memory assessment at Day 52 and were euthanized at Day 56. (a) In NOR, after initial exposure to two identical objects, mice were re-exposed 24 hours later to one novel and one familiar object, and the time spent with each was recorded and analyzed by calculation of the discrimination index (DI). A positive value indicates novelty preference and a zero value no preference. A03-treated E4FAD mice showed significant novelty preference (p = 0.0461) as compared to vehicle-treated E4FAD controls (n = 13 for NTg Veh; n = 16 for E4FAD groups). E4FAD vehicle mice showed no novelty preference. The novelty preference of A03-treated mice was equal to that of NTg Veh mice. Because the difference between NTg Veh and E4FAD Veh mice was not significant, there may be a ceiling effect, that is, a limit to the improvement that can be seen. (b) While the improvement in memory in NOR did not reach significance for either gender when analyzed separately, the means for both female and male A03-treated E4FAD were higher than those for Veh-treated E4FAD mice. (c) Exploration time – the total time spend with both objects – was similar for E4FAD Veh and A03-treated mice, suggesting treatment did not affect activity level. (d) In the Y maze FA study, there were no significant differences amongst groups in entries into the Novel Arm C (divided by all arm entries) in either minute 1 or by 5 minutes. All statistical analysis was performed using a two-tailed, unpaired t-test to compare E4FAD groups only. All data graphed as the mean ± SEM.