Figure 1 | Scientific Reports

Figure 1

From: Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects

Figure 1

Lower oleic acid accumulation and incorporation into lipids in myotubes from athletic subjects. Satellite cells isolated from biopsies from musculus vastus lateralis from athletic and sedentary subjects were cultured and differentiated into myotubes. (a) Fatty acid accumulation of [14C]oleic acid (100 µM) during the last 24 h of the differentiation period was measured with SPA. (b) Myotubes were treated with [14C]oleic acid (100 µM) for 24 h, and lipids were thereafter extracted from the cells, separated by TLC and quantified by liquid scintillation. CE, cholesteryl ester; DAG, diacylglycerol; FFA, free fatty acid; PL, phospholipid; TAG, triacylglycerol. Data are presented as means ± SEM (n = 6 in each group) in nmol/mg cell protein. *Statistically significant versus sedentary (p < 0.05, a: Linear mixed-model analysis, SPSS, b: Mann-Whitney test).

Back to article page