Table 1 Suitable oligonucleotides for the amplification of gRNA expression cassettes.
From: A novel cloning strategy for one-step assembly of multiplex CRISPR vectors
Oligonucleotide | Sequence |
|---|---|
ASAP_BbsI_fwd_A | GAGTGAAGACTTCTAGCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_B | GAGTGAAGACTTAAAAGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_C | GAGTGAAGACTTGGAAGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_D | GAGTGAAGACTTAAGGGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_E | GAGTGAAGACTTCCAAGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_F | GAGTGAAGACTTAACCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_G | GAGTGAAGACTTATGCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_H | GAGTGAAGACTTTTTTGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_I | GAGTGAAGACTTCCCCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_J | GAGTGAAGACTTGGGGGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_K | GAGTGAAGACTTGGTTGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_L | GAGTGAAGACTTCCTTGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_M | GAGTGAAGACTTTTGGGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_N | GAGTGAAGACTTTTCCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_O | GAGTGAAGACTTATCGGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_P | GAGTGAAGACTTTAGCGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_Q | GAGTGAAGACTTTACGGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_R | GAGTGAAGACTTCGATGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_S | GAGTGAAGACTTGCATGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_fwd_T | GAGTGAAGACTTCGTAGAGGGCCTATTTCCCATGAT |
ASAP_BbsI_rev_B | CTCAGAAGACAATTTTGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_C | CTCAGAAGACAATTCCGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_D | CTCAGAAGACAACCTTGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_E | CTCAGAAGACAATTGGGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_F | CTCAGAAGACAAGGTTGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_G | CTCAGAAGACAAGCATGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_H | CTCAGAAGACAAAAAAGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_I | CTCAGAAGACAAGGGGGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_J | CTCAGAAGACAACCCCGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_K | CTCAGAAGACAAAACCGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_L | CTCAGAAGACAAAAGGGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_M | CTCAGAAGACAACCAAGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_N | CTCAGAAGACAAGGAAGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_O | CTCAGAAGACAACGATGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_P | CTCAGAAGACAAGCTAGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_Q | CTCAGAAGACAACGTAGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_R | CTCAGAAGACAAATCGGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_S | CTCAGAAGACAAATGCGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_T | CTCAGAAGACAATACGGCCATTTGTCTGCAGAATTGG |
ASAP_BbsI_rev_Z | CTCAGAAGACAACTAGCGCCATTTGTCTGCAGAATTGG |
ASAP_BsmBi_fwd_A | GAGTCGTCTCTCCGGTGAGGGCCTATTTCCCATGAT |
ASAP_BsmBi_fwd_B | GAGTCGTCTCTAAAAGAGGGCCTATTTCCCATGAT |
ASAP_BsmBi_fwd_C | GAGTCGTCTCTGGAAGAGGGCCTATTTCCCATGAT |
ASAP_BsmBi_fwd_D | GAGTCGTCTCTAAGGGAGGGCCTATTTCCCATGAT |
ASAP_BsmBi_fwd_E | GAGTCGTCTCTCCAAGAGGGCCTATTTCCCATGAT |
ASAP_BsmBi_rev_B | CTCACGTCTCATTTTGCCATTTGTCTGCAGAATTGG |
ASAP_BsmBi_rev_C | CTCACGTCTCATTCCGCCATTTGTCTGCAGAATTGG |
ASAP_BsmBi_rev_D | CTCACGTCTCACCTTGCCATTTGTCTGCAGAATTGG |
ASAP_BsmBi_rev_E | CTCACGTCTCATTGGGCCATTTGTCTGCAGAATTGG |
ASAP_BsmBi_rev_Z | CTCACGTCTCACCGGTGCCATTTGTCTGCAGAATTGG |
