Figure 2
From: Multiple genetic mutations caused by NKX6.3 depletion contribute to gastric tumorigenesis
NKX6.3 regulates AICDA/APOBEC gene family expression. (A) Real-time RT-PCR (left), ChIP-qPCR (middle), and luciferase activity (right) analysis of APOBEC family, AICDA, Ung, and Apex1 in NKX6.3 depleted cells (n = 3). Error bars, SD. P values were derived from t tests. *P < 0.05, **P < 0.01. (B) Immunoblot analysis of APOBEC3A, 3B, 3C, 3G, AICDA, and Ung protein expression in NKX6.3 depleted cells. (C) Deaminase activity in HFE-145shNKX6.3 cells by FRET assay (n = 3). Error bars, SD. P values were derived from t tests. ****P < 0.0001. (D) ChIP-qPCR (upper left), luciferase activity (upper right), real-time RT-PCR (lower left), and immunoblot (lower right) analyses of CBFβ and NFκB p65 genes in NKX6.3 depleted HFE-145shNKX6.3 cells (n = 3). Error bars, SD. P values were derived from t tests. **P < 0.01; ***P < 0.001. (E) Protein expression of AICDA/APOBEC gene family in HFE-145shNKX6.3 cells with CBFβ or NFκB p65 knockdown. (F) In 3D-PCR, somatic mutation in TP53 coding region (n = 11 for shCtrl, n = 9 for shNKX6.3, n = 13 for shNKX6.3 with siCBFβ, n = 11 for shNKX6.3 with siNFκB).
