Figure 4 | Scientific Reports

Figure 4

From: Loading ceftriaxone, vancomycin, and Bifidobacteria bifidum TMC3115 to neonatal mice could differently and consequently affect intestinal microbiota and immunity in adulthood

Figure 4

Microbial community composition alterations of mice at PND21 and PND56. (a,b) Total extracted DNA of bacteria from feces was amplified by quantitative PCR and the 16S rDNA copies were calculated as the fecal bacterial numbers. The log10 value of the original data are shown (n = 8/group). (c,d) Alpha-diversity with phylogenetic diversity of intestinal microbiota is shown (n = 4/group). (e) Genus level phylogenetic classification of 16S rRNA gene frequencies in feces collected from naïve control and neonatally antibiotic/probiotic-treated animals (n = 4/group). Each bar represents one group. Rare taxa (<1%) are classified into “others”. (f) Bacterial communities from feces of naive control and neonatally antibiotic/probiotic-treated mice were compared using principal coordinate analysis (PCoA) (n = 4/group).The PCoA analysis is based on the weighted Unifrac distance. The two ends of the horizontal line represent the two compared groups, the asterisk on the line represents significant difference between groups, *p < 0.05, **p < 0.01, ***p < 0.001, for intergroup comparisons. TMC3115, Bifidobacterium bifidum TMC3115; Ceftri, Ceftriaxone; Vanco, Vancomycin. PND, postnatal day.

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