Table 3 Detection of plasmid-mediated antimicrobial resistance determinants in the 2016 WHO Neisseria gonorrhoeae reference strains (n = 14)40,41 using Whole Genome Sequence Analysis (WGSA; www.wgsa.net)21 and an in house-customised CLC Genomics Workbench. The discrepant results from the published references are highlighted in bold letters.
WHO F | WHO G | WHO K | WHO L | WHO M | WHO N | WHO O | WHO P | WHO U | WHO V | WHO W | WHO X | WHO Y | WHO Z | Incorrect calls | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
wgsa.net | Canu45 + Nanopolish | bla TEM | No | No | No | No | No | Yes | No | No | No | No | No | No | No | No | 3 |
tet(M) | No | No | No | No | No | No | No | No | No | No | No | No | No | No | 2 | ||
bla TEM | No | Yes | No | No | No | Yes | No | No | No | No | No | No | No | No | 4 | ||
tet(M) | No | No | No | No | No | No | No | No | No | No | No | No | No | No | 2 | ||
SMARTdenovo | bla TEM | No | Yes | No | No | No | Yes | No | No | No | No | No | No | No | No | 4 | |
tet(M) | No | No | No | No | No | No | No | No | No | No | No | No | No | No | 2 | ||
MaSuRCA49 | bla TEM | No | Yes | No | No | No | Yes | No | No | No | No | No | No | No | No | 4 | |
tet(M) | No | No | No | No | No | No | No | No | No | No | No | No | No | No | 2 | ||
In house-customised CLC Genomics Workbench | bla TEM | No | No | No | No | Yes | Yes | Yes | No | No | Yes | No | No | No | No | 0 | |
tet(M) | No | Yes | No | No | No | Yes | No | No | No | No | No | No | No | No | 0 | ||
