Figure 6

Ca⁺⁺-bound hTIM-3 structural comparison (PDB code 6DHB) with Na⁺-bound hTIM-3 (PDB code 5F71). An α- helix that appears only in the Na⁺-bound hTIM-3 structure instead of C″ strand is marked by an asterisk (*). The β strands are labeled with uppercase letters and loops are highlighted in italics. (a) Ribbon diagram of the 2.4 Å resolution crystal structure of hTIM-3 with bound Na⁺. (b) Surface view of Na⁺-bound hTIM-3. (c) Structural alignment between Ca⁺⁺-bound hTIM-3 crystal structure (green, PDB code 6DHB) with Na⁺-bound hTIM-3 crystal structure (orange, PDB code 5F71). (d) The lack of a C″ strand in the Na⁺-bound hTIM-3 crystal structure (orange) and appearance of an α- helix (marked by an asterisk) at this region. Ca⁺⁺-bound hTIM-3 is shown in green and C″ strand as observed in Ca⁺⁺-bound hTIM-3 crystal structure is labeled. (e) Ribbon diagram of hTIM-3 crystal structure with bound Ca⁺⁺. The loops, strand, residues and Ca⁺⁺ are colored based on B-factor range (blue-white-red, where blue minimum = 10, red maximum = 40). (f) Ribbon diagram of Na⁺-bound hTIM-3 crystal structure. The loops, strand, residues and Na⁺ are colored based on B-factor range (blue-white-red, where blue minimum = 10, red maximum = 40).