Figure 3
The non-random chromosomal distribution of microsatellites in three Karenia species. DAPI-stained DNA (blue) and in situ hybridization of the biotin-labeled (AC)10 (A), (GACA)4 (B,C) and (AG)10 (D–F) probes (red) in K. brevis (A,D), K. selliformis (B,E) and K. mikimotoi (C,F). A section of a nucleus with its chromosomes is shown at higher magnification in C; the arrows point to the band-like hybridization signals of different intensity. The AG-chromosome present in K. selliformis and K. mikimotoi is shown by the arrows in E and F, respectively. Note the absence of this chromosome in the nuclei of K. brevis cells (D). Scale bar = 10 μm, except in C (4 μm).
