Figure 2
From: Rotational direction of flagellar motor from the conformation of FliG middle domain in marine Vibrio
The biased directional rotation of fliG mutants. The fliG/pomA/pomB mutant (NMB301) harboring pNT1 with a fliG mutation and pYA303 with a wild-type (WT)-pomA and pomB was grown and observed by high-intensity dark-field microscopy. (A) The ratio of counterclockwise (CCW) rotation to CW rotation of the fliG mutants is shown in the column. All the experiments were repeated at least 6 times, and average values with standard deviation (SD) are shown. (B) The switching frequency in 10 sec was measured. All the experiments were repeated at least 6 times, and average values with SD are shown. The black bars indicate that P < 0.01 (*) for WT-FliG versus the mutants by the Welch’s t-test.
