Figure 2
From: T cell-derived lymphotoxin limits Th1 response during HSV-1 infection
The LTβR-Ig blockade results in an enhanced Th1 response in a non-viral-replication-dependent manner. (a) Viral load of peripheral nervous tissues tested on day 8 p.i. (F.P.: foot-pad; S.N.: sciatic nerves; S.C.: spinal cord. n = 4/group). (b) HSV-gE detected in the DLN (n = 4/group). (c) Th1 response in the Heat-iHSV infection model on day 14 p.i. 5 × 107 pfu of HSV-1 was heat-inactivated at 60 °C for 30 min and injected into the foot-pad. (n = 4/group), including immunospots and absolute numbers of IFNγ-secreting CD4+ cells per LN. (d) Th1 response in an OVA-CpG foot-pad vaccination model (100 μg of OVA and 50 μg of CpG-1826) on day 10 post immunization (n = 5/group), including immunospots and absolute numbers of IFNγ-secreting CD4+ cells per LN. Data are representative of three independent experiments, shown as mean ± SEM. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001. (Two-way ANOVA multiple comparisons for b).
