Figure 3
NanoCT data (A–C) in comparison with the histological microscopic slide (D) derived from the same mouse liver lobule after application of the hematein-based X-ray staining protocol. Clear visualization of the larger hepatocyte cell nuclei and the smaller cell nuclei such as Kupffer cells and SECs in white (A–C) or dark purple (D) and the BC network displayed in black (A-C) or white (D) was achieved, respectively. (A) The volume of interest (VOI) highlighting the two nanoCT slices shown in (B, blue frame) and (C, orange frame). (B, C) Representative individual nanoCT slices as indicated in the VOI from (A). (B) and (C) are positioned orthogonal to each other. The orientation of the BC network, which is formed by the hepatocytes is seen, i.e. more horizontal arrangement is seen in (B) and a more vertical alignment in (C). The nanoCT slice thickness is 580 nm. (D) Representative histological microscopic slide with a thickness of 3 µm obtained from the same mouse liver lobule sample after the applied hematein-based staining and embedding in a paraffin block.
