Figure 4
3D visualization and analysis of the different cell nuclei present within the mouse liver VOI. (A) 3D VOI showing the segmentation (inner volume and segmented cross-section of the cube surfaces) of all the cell nuclei from the original gray-value nanoCT data with hepatocytes labeled in blue and the other cells (containing Kupffer cells and SECs) highlighted in orange. (B) Entire segmentation (only inner volume without segmented cross-section of the cube surfaces) of all hepatocytes (shown in blue) and other cells such as Kupffer cells and SECs (shown in orange) is shown. (C) 3D distribution of the hepatocytes within VOI. (D) 3D distribution of all other cell nuclei within VOI. For 3D analysis the VOI has been compartmentalized in eight sub-cubes, whereby each sub-cube is visualized by dotted lines and numbered accordingly. Sub-cube one is highlighted in green for clarity. A movie is provided in the supplementary information (Movie S1).
