Table 1 Antibodies and markers used for immunolabelling studies.

From: Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep

Antibody

Concentration

Specificity

Supplier details

Calmodulin-dependent protein kinase IIα (CaMKIIα)

1:200

Excitatory neurons

#C6974; Sigma-Aldrich

CTIP2

1:500

Layer 5 cortical neurons; weaker expression in layer 6 neurons

#ab18465; Abcam

Gamma-aminobutyric acid (GAD)65/67

1:200

GABAergic interneurons

#ab49832; Abcam

Parvalbumin (PV)

1:50

PV interneuron subpopulation

#ab11427; Abcam

Myelin basic protein (MBP)

1:500

Myelin

#MBP; Aves Labs

NeuN

1:20

All post-mitotic neurons

#MAB377; Merck Millipore

Biotinylated Wisteria floribunda agglutinin (WFA)

1:400

Perineuronal nets

#L1516; Sigma-Aldrich

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