Figure 2

Multi-Angle TIRF system. (a) The opto-electronic scheme. Fibered laser bench (green) is coupled to the microscope (red) through galvanometric mirrors and two beam expanders (blue) to achieve homogeneous illumination and accurate illumination angles. All fast electronic devices are synchronized by the DAQ optimizing the acquisition rate. (b) The camera transfer time (1.5 ms) is exploited to move the galvanometric mirrors to their subsequent locations.