Figure 6 | Scientific Reports

Figure 6

From: Nanometric axial resolution of fibronectin assembly units achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy

Figure 6

Matrix synthesis. Spinning-disk imaging of endothelial cells either (a) 4 h hours after plating on fibronectin (FN) or (b) 48 h hours with no coat. F-actin was detected with phalloidin (magenta) and nuclei with DAPI (cyan). Schemes illustrate differences in the topology and thickness of fibronectin that is adsorbed to the coverslip (a) or assembled in fibrils following secretion by cells (b). (c) Correlation of altitude of α5 versus α5β1. The green contours are for cells 4 h after plating and red ones after 48 h. We measured a constant shift of 70 nm (the dashed line in c) between α5 and α5β1.

Back to article page