Figure 1 | Scientific Reports

Figure 1

From: PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia

Figure 1

The characterization of ASC-derived EVs, collected after cell stimulation with PDGF. (a,b) The representative transmission electron microscopy images of cEVs (a) and PDGF-EVs (b) negatively stained with NanoVan (black line = 500 nm); (c,d) the representative diagram of cEVs (c) or PDGF-EVs (d) according to NTA; (e) the diagram of CD63 and CD81 concentration in cEVs and PDGF-EVs according to ELISA, (mean ± SEM, *p < 0.05 vs. cEVs, n = 6); (f) the representative western blot images of CD63 and CD81 expression in ASC-derived EVs, 10 μg of protein per lane; (g) the representative image of FACS analysis of CD63 expression on cEV (left) and PDGF-EV (right); (h) the representative image of FACS analysis of CD81 expression on cEV (left) and PDGF-EV (right); (i) the representative image of FACS analysis of phosphatidylserine expression on cEV (left) and PDGF-EV (right).

Back to article page