Table 1 Successful establishment of keratinocyte cultures from cervical biopsies of phase 1.

From: Isolation of Biopsy-Derived, Human Cervical Keratinocytes Propagated as Monolayer and Organoid Cultures

Case ID

Category

Days to passage 1

Days of life span

Size of biopsy

81

0

Medium

73

1

Medium

95

0

Medium

57

1

Medium

28

3

38

60

Medium

03

0

Small

68

0

Large

96

3

31

53

Large

39

1

18

30

Small

48

1

Small

17

1

Large

22

2

28

32

Medium

97

1

Medium

27

0

Small

43

1

Medium

59

1

Large

62

0

Large

84

0

Small

04

0

Medium

15

0

Medium

  1. Cases follow in chronological order reflecting when biopsies were taken. Note that of 30 specimens acquired, only 20 are included in this table as explained in Methods. Only 3/11 specimens that initially yielded colonies were proliferating. Here, culture life span is defined as until half of the population had acquired a “fried egg” phenotype (Methods). Samples were divided into 4 categories: 0 = No colonies of keratinocytes on starting in a T12.5 flask; 1 = Visible colonies of keratinocytes but either no or too little growth to move to next size flask (i.e. from T12.5 to T25); 2 = transfer to next size flask possible (T12.5 to T25); 3 = transfer to next size flask possible (T25 to T75). Flask scaling: We typically split the T12.5 flask to the next size T25 flask once it was ~50% confluent because the centre of the colonies was typically quite dense; the T25 flask was trypsinized at ~80% confluence and all recovered cells were seeded on the subsequent T75 flask.