Figure 2

Subcellular localization of VHb, RecA, CoxIV, and AtHRS1. (A) A control protoplast expressing free GFP. (B) The same protoplast as in (A) showing chlorophyll autofluorescence in the plastids. (C) The same protoplast as in (A) under white light. (D) A merge of images shown in (A,B). (E) Green fluorescence from the chloroplasts of a protoplast expressing pVHb-RecA. Protoplasts that expressed free GFP emitted green fluorescence signals similar to that in the controls. (F) The same protoplast as in (E) showing red fluorescence. (G) The same protoplast as in (E) under white light. (H) A merge of images shown in (E,F). (I) Green fluorescence from the mitochondria of a protoplast expressing pVHb-CoxIV. (J) The same protoplast as in (I) showing chlorophyll autofluorescence in the plastids. (K) The same protoplast as in (I) expressing a mitochondrion-localizing F1ATPase-γ-RFP giving a red fluorescence signal. (L) A merge of images from (I) to (K). (M) Green fluorescence from the chloroplasts and mitochondria of a protoplast expressing pVHb-AtHRS1. (N) The same protoplast as in (M) showing chlorophyll autofluorescence in the plastids. (O) The same protoplast as in (M) expressing a mitochondrion-localizing F1ATPase-γ-RFP giving a red fluorescence signal. (P) A merge of images from (M–O). (Q) Green fluorescence from the cytoplasm of a protoplast expressing pVHb. (R) The same protoplast as in (Q) showing chlorophyll autofluorescence in the plastids. (S) The same protoplast as in (R) expressing a mitochondrion-localizing F1ATPase-γ-RFP giving a red fluorescence signal. (T) A merge of images from (Q–S). Bars = 7.5 μm.