Figure 2 | Scientific Reports

Figure 2

From: Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates

Figure 2

Characterization of poly-protein G-expressing cells. (A) The expression of protein G or poly-protein G in BALB/c 3T3 cells was analyzed by western blot using a mouse anti-HA tag antibody. Lane 1, BALB/c 3T3 cells as negative control; Lane 2, single protein G C2 domain expressing BALB/c 3T3 cells (1pG cells); Lane 3, eight tandemly repeated C2 domain expressing BALB/c 3T3 cells (8pG cells). (B) The surface displays of functional protein G (middle) and poly-protein G (right) were analyzed by flow cytometry using a FITC-conjugated goat antibody.

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