Figure 2 | Scientific Reports

Figure 2

From: Modulation of benzylisoquinoline alkaloid biosynthesis by overexpression berberine bridge enzyme in Macleaya cordata

Figure 2

(A–D) GUS assay of leaf and stem section of transgenic and non-transgenic M. cordata plants. (A,B) Staining of GUS activity in overexpression McBBE-OX plants. (C) Staining of GUS activity in transgenic PsBBE-OX plant. (D) Staining of GUS activity in non-transgenic plant. (E–G) Amplify of GUS, nptII, PsBBE genes in transgenic and non-transgenic plants, marker 2 kb plus ladder and vector1 (pCMBIA-2301), vector2 (pCMBIA-2301 + PsBBE), vector3 (pCMBIA-2301 + McBBE) were used to positive control, WT negative control. (E) Amplify GUS gene (750 bp). (F) Amplify nptII gene (364 bp). (G) Amplify PsBBE gene (750 bp). Vec1-Vec3 means vector1 to vector3. WT means wild type.

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