Figure 2

Enzyme kinetics and MS spectrum for product pattern. (A) Kinetics of CSN-MN-catalyzed cleavage of a chitosan polymer with a DA of 10%. Kinetics data showed a sigmoidal curve of the Hill reaction, where K_M was 0.74 mg/ml and kcat was 163 s⁻¹, with a Hill coefficient of 1.54. Data are means of twelve (n = 12) independent experiments ± s.d. (B) Mass spectrum of oligomeric products obtained by CSN-MN-catalyzed hydrolysis of a chitosan polymer with a DA of 30%; D, GlcN; A, GlcNAc. The major products identified after hydrolysis were GlcN₃, GlcN₂GlcNAc₁, GlcN₃GlcNAc₁, and GlcN₄GlcNAc₁ (see also Fig. S3). Data shown are representative of results obtained in three independent experiments.