Figure 5
Comparison of induction of HIF-1α and GLUT-1 between LoVo and HT29 cells/tumor tissues. (A) Micrographs of immunofluorescence staining for nuclei (blue) and HIF-1α/GLUT-1 (green) in LoVo (upper panel) and HT29 (lower panel) cells (magnification, 200×). (B) and (C) Quantification of HIF-1α and GLUT-1 staining using Live cell Imaging System. (D) Immunohistochemical staining for GLUT-1/HIF-1α (brown) in LoVo (upper panel) and HT29 (lower panel) xenograft tumor tissues (magnification, 200×), respectively. (E) Western blot analyses of HIF-1α and GLUT-1 protein expression in LoVo (right lane) and HT29 (left lane) xenograft tumor tissues. (F) Quantification of immunohistochemical staining. (G) Quantification of Western blot analyses. ***P < 0.0001, **P < 0.001, *P < 0.05, n = 3.
