Figure 3

Cigarette smoking cessation is associated with normalization of ABC transporter gene expression patterns in airway epithelial cells. (a) Differential expression of ABCA13 between current smokers (green) and former smokers (blue) from GSE37147 (* q < 0.05, Mann-Whitney U pairwise statistical analysis with Benjamini Hochberg multiple testing correction). GSE37147 was selected as it was the only dataset including a probe matching ABCA13. (b–d) Differential expression of ABCB6, ABCC1, and ABCC3 between current smokers (green), former smokers (blue), and, where possible, never-smokers (orange) from GSE37147 and GSE994 (* q < 0.05, Kruskal-Wallis followed by the Tukey Honest Significance Difference (Tukey HSD) test). The datasets used in this analysis (GSE37147 and GSE994) were generated from two distinct microarray platforms (Affymetrix Human Gene 1 ST and Affymetrix Human Genome U133A, respectively), which analyzed epithelial cells from medium (6^th-8^th generation) and large (2^nd generation) airways, respectively. GSE37147 used the logged RMA Normalization method and GSE994 used the Mas 5.0 Normalization method. Data distributions are visualized as boxplots (see Methods for additional details).