Figure 4

Detection of flavonoid and total sericin in the cocoon of transgenic silkworm. (A) Phenotype of cocoons. (B) Diameter of silk fibres produced by wandering-stage silkworms. (C) Detection of compounds in the cocoon by HPLC (C1, flavonoid extract solution; C2, analysis of flavonoid extract solution by HPLC; C3, overlapping peaks in the chromatogram). (D,E) Quantitative analysis of quercetin/rutin content in cocoons by HPLC. (F) Analysis of total sericin content in cocoons by SDS-PAGE.