Table 1 Primers used in this study.
Gene | Genbank Accession No. | 5′-3′ | Purpose | Sequence | Remark |
|---|---|---|---|---|---|
GAL4 | AB186055.1 | F | RT-PCR | GCAATCCCATTACCGCATATC | 431 bp |
R | AGGTTCGGACCGTTGCTACTGT | ||||
EGT | AY048771.1 | F | CCAGCGTCCAGTATCTTG | 649 bp | |
R | CGACGACTGATGTCATGTC | ||||
BmActin3 | AB701689.1 | F | AACACCCCGTCCTGCTCACTG | 675 bp | |
R | GGGCGAGACGTGTGATTTCCT | ||||
BmLP3 | FJ214661.1 | F | For vector construction in transgenic larvae | GCgtcgacAGTATAGTTACAACGGCTGCCCC | 1124 bp |
R | CGggatccGGAGCGTCGAGTCCTGCAATATG | ||||
GAL4 | AB186055.1 | F | CGggatccATGAAGCTACTGTCTTCTATCG | 2646 bp | |
R | TTgcggccgcTTACTCTTTTTTTGGGTTTGGTGG |
