Figure 2

Xrp1 is required for the elimination of RpL19^+/− loser cells. Schematic representation of the twin spot MARCM system used to generate RpL19^+/− loser clones (red) and their respective twin spot clones (bright green, two copies of GFP) in a background of wild-type GFP positive cells (dark green) (A). RpL19^+/− loser cells are eliminated (B). RpL19^+/− cells are eliminated when Xrp1 mutations are rescued with the re-introduction of one copy of Xrp1 (B’). Loser cells elimination is rescued via either intronic Xrp1^08−/− mutations retrieved from the EMS screen (B”) or, even more efficiently, via Xrp1^61−/− mutations in the coding sequence of Xrp1 (B”’). Quantification of the mean ratio between mCherry Area and GFP²⁺ area (mChe/GFP). Minute loser cells, labeled with mCherry, are eliminated and the mChe/GFP ratio is close to 0. Xrp1 mutants rescue the elimination of loser cells (ratio close to 1). ***P < 0.001, *P < 0.05, Kruskal-Wallis test. Bars represent SEM. n = 52,47,45,48. Additional significance was calculated via assessing distribution normality (D’Agostino & Pearson normality test). Xrp1^08−/− and Xrp1^61−/− follow a normal distribution (P < 0.001) (C).