Figure 4 | Scientific Reports

Figure 4

From: Proper direction of male genitalia is prerequisite for copulation in Drosophila, implying cooperative evolution between genitalia rotation and mating behavior

Figure 4

Analysis of courtship behavior using a novel image-processing program. (a) A snapshot of the video data of mating behavior between a virgin wild-type female and a male with genitalia with angle deviation or a control male with genitalia in the normal dorsoventral direction. (b and c) Snapshot of the original video (b) and its corresponding binarized picture (c) from the video recording of mating behavior in a single chamber. (d) Processed image in which the binarized images were automatically arranged in 3D space along a time line. A moving bar (yellow line) that was synchronized with the 2D image in b, c was inserted into the 3D image. Black triangles show time points.

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