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Figure 3

From: Experimental Metabolic Syndrome Model Associated with Mechanical and Structural Degenerative Changes of the Aortic Valve

Figure 3The alternative text for this image may have been generated using AI.

Masson’s Trichrome, Elastin, and Movat’s Pentachrome Histology Distinguishing Collagen and Elastic Fibers in Normal Diet Versus High-fat Diet Pigs After 16 weeks (n = 10). (A) Masson’s trichrome: Collagen components observed in normal diet pigs showing the collagenous architecture. (B) Increased collagen concentration observed in the HF diet pigs at the fibrosa layer (denoted in red arrow) of the aortic cusps. The collagen threshold area is two-times greater in the HF diet group compared to the normal diet group. This was measured semi-quantitatively by percentage area of collagen on Masson’s Trichrome. (C) Elastin fibers (denoted with yellow arrow) found within the normal diet group aortic cusp ECM. (D) There were no observable elastin fibers present in the high fat diet group due to the pathological collagen remodeling. (E) Movat’s Pentachrome: Normal Diet cusp show uniform ECM architecture containing ground substance and mucins. (F) Pigs administered a HF diet showed an increase of collagen within the ECM (denoted with black arrows) in the fibrosa and spongiosa layers of the aortic cusps. (G) Quantitative Analysis of Collagen Types 1 and 3 Utilizing ELISA in Normal Diet Versus High Fat Diet Pigs After 16 Weeks (n = 12). Collagen type 1 concentration is increased in HF diet compared to the normal diet group (P < 0.01). Collagen type 3 concentration is increased in HF diet compared to the normal diet group (P < 0.01).

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