Figure 1
From: Biomechanical characterization of TIM protein–mediated Ebola virus–host cell adhesion
Schematic of the experimental system. (A) The AFM cantilever is functionalized with the PS, VLP, or EBOV GP/VSV. TIM-1 or TIM-4 is immobilized on the opposing glass surface. (B) The upper panel shows three sample AFM pulling traces of the TIM-4–PS interaction. The first (upper) trace involves no interaction. The second (middle) trace shows the rupture force of the TIM-4–PS complex. The lower trace shows a typical non-specific interaction. Fu is the unbinding force. ks is the system spring constant and was derived from the slope of the force-displacement trace. The cantilever retraction rate of the measurements was 1.5 μm/s. The lower panel illustrates the four stages of stretching and rupturing a single ligand-receptor complex using AFM. (C) A schematic diagram of the live-cell AFM assay. Insert: micrograph of a VLP -functionalized cantilever showing GFP fluorescence. Bar is 20 µm.
