Table 1 Strains and plasmids used in this study.
Strains or plasmids | Genotype/Description | Sources |
|---|---|---|
Strains | ||
E. coli DH5α | F − recA endA1 Φ80dlacZΔM15 hsdR17(r K − m K + ) λ − | Invitrogen |
E. coli BL21(DE3) | F− ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) | Invitrogen |
S. cerevisiae 288c | MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6 | American type culture collection (ATCC) |
B. trispora DSM-2388 | Mating type - | German Collection of Microorganisms and Cell Cultures (DSMZ) |
Plasmids | ||
pACYCDuet-1 | Cm r oriP15A lacI q T7p | Novagen |
pET30a(+) | Kan r oripBR322 lacI q T7p | Novagen |
pEASY-Blunt | Kan r Amp r oripUC | Transgen |
pTrcHis2B | Amp r ori pBR322 lacI q Trcp | Invitrogen |
pUC-lgfS | pUC57 harboring codon optimized P. abies lgfS | This study |
pET-lgfS | pET30a harboring P. abies lgfS | This study |
pA-lgfS | pACYCDuet-1 harboring P. abies lgfS | This study |
pEasy-ispA | pEASY-Blunt harboring E. coli ispA | This study |
pEasy-isoA | pEASY-Blunt harboring B. trispora isoA | This study |
pEasy-erg20 | pEASY-Blunt harboring S. cerevisiae erg20 | This study |
pA-ispAlgfS | pACYCDuet-1 harboring E. coli ispA and P. abies lgfS | This study |
pA-isoAlgfS | pACYCDuet-1 harboring B. trispora isoA and P. abies lgfS | This study |
pA-erg20AlgfS | pACYCDuet-1 harboring S. cerevisiae erg20 and P. abies lgfS | This study |
pTrcLower | pTrcHis2B harboring S. cerevisiae erg19, erg8, erg12 and idi1 | |
pA-mvaES | pACYCDuet-1 harboring E. faecalis mvaE and mvaS | |
pA-mvaESispA | pACYCDuet-1 harboring E. faecalis mvaE, mvaS and E. coli ispA | This study |
pA-mvaESispAlgfS | pACYCDuet-1 harboring E. faecalis mvaE, mvaS, E. coli ispA and P. abies lgfS | This study |
