Table 2 Primers used in this study for plasmids construction.

From: Manipulation of the precursor supply for high-level production of longifolene by metabolically engineered Escherichia coli

Oligonucleotide primers

Sequences

lgfS_F_NdeI

GGGAATTCCATATGGCTCAGATCAGTAAATGCTC

lgfS_R_BglII

GGAAGATCTTAGGTCAGCGGGTCGATCAG

ispA_F_NcoI

CCATGGACTTTCCGCAGCAACTC

ispA_R_BamHI

GGATCCTTATTTATTACGCTGGATGATGTAGTCC

isoA_F_NcoI

CCATGGTTGCTGTCAAGTTACCAAAG

isoA _R_SalI

GTCGAC TTATTTAGTACGCTTGTAGATCTTG

erg20_F_NcoI

CCATGGCTTCAGAAAAAGAAATTAGGAG

erg20_R_BamHI

GGATCCCTATTTGCTTCTCTTGTAAACTTTGTTC

ispA_F_BglII

GGAAGATCTCATGGACTTTCCGCAGCAACTC

ispA_R_PvuI

TCGCGATCGTTATTTATTACGCTGGATGATGTAGTCC

T7lgfS_F_PvuI

TCGCGATCGTAATACGACTCACTATAGGGGAATTGTG

T7lgfS_R_XhoI

CCGCTCGAGTTAGGTCAGCGGGTCGATCAGGATTTTG

  1. The restriction sites in the primers were underlined.
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