Figure 5 | Scientific Reports

Figure 5

From: Effective CRISPR/Cas9-mediated correction of a Fanconi anemia defect by error-prone end joining or templated repair

Figure 5

Activation of the FA pathway after CRISPR/Cas9 templated repair in mouse ES cells. Western blot to detect short and long forms of FANCD2 in parental and template corrected mESCs. Upon exposure to hydroxy urea (HU) the short (S) from of FANCD2 becomes the long (L) form due to the ligation of a mono-ubiquitin group mediated by a functional FA core complex. The ESC corrected by Cas9D10A revealed one template repaired allele and one c.828InsTAAA allele, while the Cas9 WT ESC appeared homozygous for templated repair.

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