Table 1 Predicted off-target sites for sgRNA + 1.

From: Effective CRISPR/Cas9-mediated correction of a Fanconi anemia defect by error-prone end joining or templated repair

Gene

Chr

Target sequence

PAM

sgRNA + 1

  

GTCTGTGTAGGGACTTTACC

On-target:

Fanconi anemia group F (Fancf)

7

A------------------- AGG

Off-target

Apoptosis-associated tyrosine kinase (Aatk)

11

G---C--------------- CA-

Off-target

Podocalyxin-like 2 (Podxl2)

6

-AG-------------G--- T--

Off-target

Amyloid β precursor protein binding (Apba1)

19

GC----CA------------ -A-

  1. The similarity between the sequences of the sgRNA + 1 coding sequence and on and off target sites is depicted. Additional information on putative off target genes is supplied in the supplemental data. Dashes indicate identical nucleotides between the sgRNA + 1 coding sequence and genomic DNA.
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