Figure 1
From: Phase-specific functions of macrophages determine injury-mediated corneal hem- and lymphangiogenesis
Time course of corneal macrophage infiltrate and lymphangiogenesis after incision injury. (A) After incision injury (day 0), the inflammatory macrophage infiltrate, lymphatic vessels (LV) and blood vessels (BV) were monitored at indicated time points. (B,C) Quantification of total corneal area covered by macrophages and LV at indicated time points. Values were compared to uninjured controls (n = 8–10 per time point). (D–L) presentative sectors of corneal whole mounts stained for F4/80 (D,G,J), LYVE-1 (E,H,K), and CD31 (F,I,L) at indicated time points after incision injury. Dashed line depicts the corneal limbus. Macrophage numbers in the cornea peak at day 7, and quickly decline. Macrophage numbers are no longer elevated on day 21. LV reach their maximum density on day 7 and start to regress until day 21 when lymphangiogenesis reaches baseline level. Corneal hemangiogenesis is not induced.
