Figure 6

Tupanvirus upregulates the expression of viral and cellular mannose binding protein (MBP) genes. A. castellanii cells were infected with tupanvirus and collected at 1, 2, 4, 6 and 8 h.p.i. and MBP mRNA levels were measured by qPCR: cellular (A) and viral (B) genes with and without the presence of mannose. The data were calculated using the 2−(Δct) method and are shown as the standard deviation of two independent biological assays. Error bars indicate SDs. The statistical significance was calculated using a two-tailed 2-way ANOVA test, Tukey’s range test, using GraphPad Prism. ****p < 0.0001.