Figure 2 | Scientific Reports

Figure 2

From: Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Figure 2

Confirmation of chromophore maturation by mass spectrometry. A comparison of GFP 1–10 before (a) and after (b) maturation according to the protocol shows a 21 Da (20.77) reduction in mass (white = hydrogen, grey = carbon, blue = nitrogen and red = oxygen). This corresponds to the loss of one oxygen and five hydrogen atoms resulting from the cyclization and covalent rearrangement during the chromophore maturation process.

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